WebbThermo Scientific Phire Hot Start II PCR Master Mix is convenient 2X mix designed to minimize the number of pipetting steps. ... Phire Green Hot Start II DNA Polymerase. Phire Hot Start II DNA Polymerase; 5X Phire Green Reaction Buffer (provides 1.5 mM MgCl 2 in the final 1X concentration) DMSO . WebbThe Phire Tissue Direct PCR Master Mix employs Phire Hot Start II DNA Polymerase, a specially engineered enzyme with a DNA-binding domain that enhances the processivity of the polymerase. ... 2x Phire Tissue Direct PCR Master Mix 2 × 1.25 mL 10 × 1.25 mL Dilution Buffer 5 mL 2 × 12.5 mL DNARelease Additive 3 × 100 µL 1.3 mL
Agarose gel electrophoresis of the PCR products obtained from multi …
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Phire Green Hot Start II PCR Master Mix
WebbThermo Scientific Phire Hot Start II DNA Polymerase is a novel PCR enzyme for routine and high throughput PCR applications. It outperforms every Taq -based hot start polymerase on the market. Phire Hot Start II … WebbThermo Scientific Phire Hot Start II PCR Master Mix is convenient 2X mix designed to minimize the number of pipetting steps. The master mix contains Phire Hot Start II DNA … WebbPCR amplification: DNA polymerase: 2× KAPA HiFi HotStart ReadyMix: Phusion Green Hot Start II High-Fidelity Master Mix: Phire Hot Start II DNA Polymerase: PCRBIO HIFI polymerase: PCR cycling conditions: 95 °C 3 min, 35× (98 °C 30 s, 57 °C 30 s, 72 °C 30 s), 72 °C 1 min: 98 °C 2 min, 30× (98 °C 15 s, 52 °C 30 s, 72 °C 30 s), 72 °C 3 min how much robux is 800 dollars