Hif1a antibody
WebHIF-1 alpha antibody used for WB on lysates of HUVEC/human umbilical vein endothelial cells after exposure to 2 Gy of IR with or without 2 day pre-incubation with … Web13 de mai. de 2024 · DNA was immunoprecipitated from the sonicated cell lysates using anti-HIF1A antibody (Abcam) and immunoprecipitates were recovered by addition of Salmon Sperm DNA/Protein A Agarose-50% Slurry.
Hif1a antibody
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WebThe hypoxia-inducible transcription factor 1 alpha (HIF-1 alpha ) is the regulated member of the transcription factor heterodimer HIF-1. HIF-1 binds to hypoxia-response … WebRabbit anti-HIF1-alpha Antibody, Affinity Purified (alpha subunit (basic helix-loop-helix transcription factor) ); Powered by Bethyl Laboratories, Inc.; Suitable for immunohistochemistry; KR EN. Applications Products Services Support. PLA0081; All Photos (3) PLA0081.
WebHIF1A Antibody (PA5-85494) in ICC/IF. Immunofluorescence analysis of Hypoxia-inducible factor 1-alpha was performed using 70% confluent log phase MCF7 cells treated with 150 µM of Cobalt chloride for 16 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with ... WebDetection of human HIF1 alpha by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from 293T cells that were treated with 200 µM cobalt chloride.Antibodies: Affinity purified rabbit anti-HIF1 alpha antibody A300-286A (lot number A300-286A-6) used for WB at 1 µg/ml and for IP at 6 µg/mg lysate.Detection: …
WebThis antibody does not cross-react with ARNT or the related HIF-2 alpha. MA1-516 has been successfully used in Western blot, immunofluorescence, immunoprecipitation, immunocytochemistry, and gel shift procedures. By Western blot, this antibody detects an ∽116 kDa protein representing HIF-1 alpha after hypoxic induction in COS cells.
Web3 de out. de 2024 · After 3 washes, they were incubated with anti-Ki67 (1:25, Abcam, Cat #ab 1667), anti-CD31 antibody (1:25, BD Biosciences, Cat #550274) at 4° C. overnight and were then incubated with either Alexa 647-conjugated anti-rabbit IgG (Life Technology), or Alexa 594-conjugated anti-rabbit IgG or Alexa 488 conjugated anti-rat IgG at room …
WebI am doing HIF1 westerns in HIF-overexpressing mouse liver and adipose tissue using Novus antirabbit HIF1a antibody with overnight incubation. I am getting strong bands around 90kDa. I am aware that HIF theoretical molecular weight is 93kDa, but in westerns, the HIF band is usually around 120kDa according to my internet research. how are espresso beans differentWebShowing subcellular location of HIF1A (bHLHe78, HIF-1alpha, HIF1, MOP1, PASD8). We use cookies to enhance the usability of our website. ... Location antibody staining i. Location tagged protein i HPA000907: HeLa: 5803: N-terminal: Nucleoplasm, Plasma membrane: Nucleoplasm, Plasma membrane ... how are essential oils toxic to dogsWebUnder normoxic conditions HIF-1 alpha has a short half-life. It is largely undetectable in cells or tissues grown under normoxic conditions. It is stabilized only at O 2 concentrations below 5% and upon stabilization under hypoxic conditions HIF-1 translocates to the nucleus. Therefore we recommend western blots using nuclear extracts and running Hypoxia … how are essential oils producedWebMonoclonal Anti-HIF-1α antibody produced in mouse (Anti-Hypoxia Inducible Factor 1, α subunit); ~ 1mg/mL, monoclonal H1α67, purified immunoglobulin, buffered aqueous solution; ... Anti-HIF1A. affinity isolated antibody. View … how many major league playersWebMonoclonal Antibody for studying HIF1A. Cited in 220 publications. Validated for WB, ChIP, ChIP, C&R. Highly specific and rigorously validated in-house, HIF-1α (D2U3T) Rabbit Monoclonal Antibody (CST #14179) … how are esters createdWebMonoclonal Antibody for studying HIF1A. Cited in 31 publications. Validated for WB, IP, IF, F. Available in 2 sizes. Highly specific and rigorously validated in-house, HIF-1α (D5F3M) Mouse Monoclonal Antibody (CST #79233) is ready to ship. how are esg ratings calculatedWebHIF1a/HIF-1 alpha antibody used for WB on proteins extracts from human lymphoblasts cultured under normoxic and hypoxic conditions. Lymphoblasts were from six patients … how are essays made