Fitc dmso
WebAug 5, 2013 · The FCS study reveals a change of the hydrodynamic radius of BSA from 3.7 nm in the native state to 7.0 nm in the presence of 40% DMSO, which suggests complete unfolding of the protein under these conditions. Fluorescence self-quenching of FITC has been exploited to understand the conformational dynamics of BSA. http://www.rx-8.com/chanpinzhongxin/206641.html
Fitc dmso
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WebFITC, 1g . FITC, 100mg . Chemical name: Fluorescein isothiocyanate: Molecular weight: 389.38 . Extinction coefficient: 70,000M-1: cm-1 ... Dissolve TRITC in DMSO at 1mg/mL. 2. While stirring, slowly add 35µl of TRITC to 1mL of the 6mg/mL antibody solution and mix thoroughly. 2. Incubate for 2 hours at room temperature in the dark.
WebDec 12, 2024 · Dissolve FITC in anhydrous DMSO at 1 mg/ml concentration. This should be prepared fresh for each labelling reaction. Add 5 μl of the dye to the dialysed protein (5 ml) every 30 min to get a final concentration of 100 ng FITC/1 μg protein. Perform this step by gently rocking the protein solution at 4°C for 12 h. Web1. Dissolve 200 µg of FITC in 200 µl of conjugation buffer and immediately mix it with 1 ml of streptavidin solution. Note: When substituting TRITC for FITC, a similar protocol can be …
WebFITC (Fluorescein isothiocyanate) is the form of fluorescein used for conjugation to all JIR antibodies and purified proteins, with the exception of streptavidin. Fluorescein … WebThey suggest to prepare protein solution not less than 2 mg/ml and add 50 ul of FITC (1 mg/ml in DMSO) per 1 ml of protein solution. Final molar F/P ratio was 0.06. In another …
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Web西安齐岳生物科技有限公司生产销售“磷脂-聚乙二醇-荧光素 dspe-peg2000-fitc”-“产品名称 磷脂-聚乙二醇-荧光素 dspe-peg-fitc 中文名称 荧光素聚乙二醇磷脂 磷脂peg荧光素 英文名称 fitc-peg-dspe dspe-peg-fitc 溶解度 溶于dmso dmf dcm等有机溶剂”，该产品仅用于科研，如果需要请联系我们 taruklohuWebAug 1, 2024 · For DMSO/DBTD method, the fluorescent label is tagged to the hydroxyl group of AX. FITC can be grafted onto AX through thiocarbamoyl linkage, as shown in Fig. 1 a. FITC reacts with AX in DMSO at elevated temperature to give O-(fluoresceinyl-thiocarbamoyl) AX with pyridine and DBTD as the catalysts. During the reaction, pyridine … 高校講座 家庭総合 ライブラリーWeb瑞禧生物提供科研级FITC-PEG(3)-NH2,我们一直致力于研发纳米靶向科研产品以满足国内广大科研院校及其他相关科研单位的需求。 ... Fluorescein-PEG3-Amine 外观：Liquid 分子量：581.638 溶解度：DMSO CAS号：1807539-04-3 质量控制：95% 储存条件：-20 ... tarukiranaWebAttach a 20-gauge needle to the tip of a 1 mL syringe. Use this syringe to extract the desired volume of DMSO (use 100 µL per mg of NHS-ester modification solid; depending on the modification, additional DMSO may be necessary). Use the “balloon” technique to maintain the DMSO stock bottle under nitrogen pressure and thereby keep moisture out. 高校 部活 うるさいThen, use anhydrous DMSO to dissolve FITC at 1 mg/ml. Do not use stored solutions of FITC in DMSO; prepare fresh on the day of the reaction. Step 2. Incubate Protein with FITC. Slowly and gently add 50 ul of FITC solution to each ml of protein solution in 5 ul intervals while stirring the mixture. Incubate the solutions in the dark at 4 °C for ... 高校通学リュック男子WebFITC-xtra conjugates are significantly brighter than FITC conjugates and are much more photostable. Additionally, the fluorescence of FITC-xtra is not affected by pH (4-10). Cat No. 135 ... DMSO: Spectral properties. Excitation (nm) 498: Emission (nm) 526: Storage, safety and handling. H-phrase: H303, H313, H333: Hazard symbol: XN: Intended use ... taru kiranaWebASHBURN (HQ) 44320 Premier plaza, Suite 210, Ashburn, VA 20147 Phone: 1-703-263-0855 Email: [email protected] taru keronen